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basic nucleofector kit for primary mammalian fibroblasts  (Lonza)


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    Structured Review

    Lonza basic nucleofector kit for primary mammalian fibroblasts
    Basic Nucleofector Kit For Primary Mammalian Fibroblasts, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/basic+fibroblast+nucleofector+kit/4d+nucleofector/10__1096_slash_fj__202500506r-67-15-17
    Average 90 stars, based on 1 article reviews
    basic nucleofector kit for primary mammalian fibroblasts - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Virus:

    Article Title: Inverse agonistic anti-US28 antibodies
    Article Snippet: BAC DNA was isolated using NucleoBond Xtra BAC kit (Macherey Nagel, Duren, Germany). .. Virus production was initiated by electroporation of BAC DNA into HFFF TR cells using the Amaxa Nucleofector and the basic fibroblast nucleofector kit (Lonza, Basel, Switserland). ..

    Electroporation:

    Article Title: Inverse agonistic anti-US28 antibodies
    Article Snippet: BAC DNA was isolated using NucleoBond Xtra BAC kit (Macherey Nagel, Duren, Germany). .. Virus production was initiated by electroporation of BAC DNA into HFFF TR cells using the Amaxa Nucleofector and the basic fibroblast nucleofector kit (Lonza, Basel, Switserland). ..

    BAC Assay:

    Article Title: Inverse agonistic anti-US28 antibodies
    Article Snippet: BAC DNA was isolated using NucleoBond Xtra BAC kit (Macherey Nagel, Duren, Germany). .. Virus production was initiated by electroporation of BAC DNA into HFFF TR cells using the Amaxa Nucleofector and the basic fibroblast nucleofector kit (Lonza, Basel, Switserland). ..

    Plasmid Preparation:

    Article Title: Establishment of an induced pluripotent stem cell line from a female domestic ferret (Mustela putorius furo) with an X chromosome instability.
    Article Snippet: Following skin biopsy of a female neonatal ferret, fibroblasts were expanded on a 0.1% gelatin-coated tissue culture dish in a fibroblast medium M10 consisting of Dulbecco’s Modified Eagle Medium (Nacalai Tesque) supplemented with 10% inactivated fetal bovine serum and 1% Penicillin/Streptomycin solution (P/S) (Thermo Fisher Scientific). .. For vector transfection, sub-confluent fibroblasts were detached using Trypsin/EDTA solution (Nacalai Tesque), and electroporated using the Nucleofector 2b (Lonza) and Basic Fibroblast Nucleofector Kit (Lonza) as described previously (Yoshimatsu et al., 2021). ..

    Article Title: Non-viral derivation of a transgene-free induced pluripotent stem cell line from a male beagle dog.
    Article Snippet: The fibroblasts were expanded on a 0.1% gelatin-coated tissue culture dish or plate in a fibroblast medium (named M10 medium) consisting of Dulbecco’s Modified Eagle Medium supplemented with 10% inactivated fetal bovine serum and 1% Penicillin/Streptomycin solution (P/S) (all purchased from Thermo Fisher). .. For vector transfection, the Nucleofector 2b (Lonza) and Basic Fibroblast Nucleofector Kit (Lonza) were used as described previously (Yoshimatsu et al., 2021). .. In brief, a total 3.65 μg DNA vectors consisting of 0.47 μg pCE-hOCT3/4 (Addgene #41813), 0.47 μg pCE-hSK (Addgene #41814), 0.47 μg pCE-hUL (Addgene #41855), 0.47 μg pCE-mp53DD (Addgene #41856), 0.47 μg pCXLEEGFP (Addgene #27082), 0.47 μg pCE-K2N (Addgene #154879), 0.47 μg pCE-KdGl (Addgene #154880) and 0.36 μg pCXWB-EBNA1 (Addgene #37624) were introduced into 1 × 106 fibroblasts using the U-23 condition.

    Article Title: Establishing an induced pluripotent stem cell line from neonatal common marmoset fibroblasts by an all-in-one episomal vector approach.
    Article Snippet: The fibroblasts were expanded on a 0.1% gelatin-coated tissue culture dish or plate in M10 medium consisting of Dulbecco’s Modified Eagle Medium supplemented with 10% inactivated fetal bovine serum and 1% Penicillin/Streptomycin solution (P/S) (all purchased from Thermo Fisher). .. For vector transfection, the Nucleofector 2b (Lonza) and Basic Fibroblast Nucleofector Kit (Lonza) were used as described previously (Yoshimatsu et al., 2021). .. In brief, a total of 3.65 μg DNA vectors consisting of 3 μg pMasterK and 0.65 μg pCXWB-EBNA1 (Addgene #37624) was introduced into 1 × 106 fibroblasts using V-13 condition.

    Transfection:

    Article Title: Establishment of an induced pluripotent stem cell line from a female domestic ferret (Mustela putorius furo) with an X chromosome instability.
    Article Snippet: Following skin biopsy of a female neonatal ferret, fibroblasts were expanded on a 0.1% gelatin-coated tissue culture dish in a fibroblast medium M10 consisting of Dulbecco’s Modified Eagle Medium (Nacalai Tesque) supplemented with 10% inactivated fetal bovine serum and 1% Penicillin/Streptomycin solution (P/S) (Thermo Fisher Scientific). .. For vector transfection, sub-confluent fibroblasts were detached using Trypsin/EDTA solution (Nacalai Tesque), and electroporated using the Nucleofector 2b (Lonza) and Basic Fibroblast Nucleofector Kit (Lonza) as described previously (Yoshimatsu et al., 2021). ..

    Article Title: Non-viral derivation of a transgene-free induced pluripotent stem cell line from a male beagle dog.
    Article Snippet: The fibroblasts were expanded on a 0.1% gelatin-coated tissue culture dish or plate in a fibroblast medium (named M10 medium) consisting of Dulbecco’s Modified Eagle Medium supplemented with 10% inactivated fetal bovine serum and 1% Penicillin/Streptomycin solution (P/S) (all purchased from Thermo Fisher). .. For vector transfection, the Nucleofector 2b (Lonza) and Basic Fibroblast Nucleofector Kit (Lonza) were used as described previously (Yoshimatsu et al., 2021). .. In brief, a total 3.65 μg DNA vectors consisting of 0.47 μg pCE-hOCT3/4 (Addgene #41813), 0.47 μg pCE-hSK (Addgene #41814), 0.47 μg pCE-hUL (Addgene #41855), 0.47 μg pCE-mp53DD (Addgene #41856), 0.47 μg pCXLEEGFP (Addgene #27082), 0.47 μg pCE-K2N (Addgene #154879), 0.47 μg pCE-KdGl (Addgene #154880) and 0.36 μg pCXWB-EBNA1 (Addgene #37624) were introduced into 1 × 106 fibroblasts using the U-23 condition.

    Article Title: Establishing an induced pluripotent stem cell line from neonatal common marmoset fibroblasts by an all-in-one episomal vector approach.
    Article Snippet: The fibroblasts were expanded on a 0.1% gelatin-coated tissue culture dish or plate in M10 medium consisting of Dulbecco’s Modified Eagle Medium supplemented with 10% inactivated fetal bovine serum and 1% Penicillin/Streptomycin solution (P/S) (all purchased from Thermo Fisher). .. For vector transfection, the Nucleofector 2b (Lonza) and Basic Fibroblast Nucleofector Kit (Lonza) were used as described previously (Yoshimatsu et al., 2021). .. In brief, a total of 3.65 μg DNA vectors consisting of 3 μg pMasterK and 0.65 μg pCXWB-EBNA1 (Addgene #37624) was introduced into 1 × 106 fibroblasts using V-13 condition.

    Knock-In:

    Article Title: Research Note: Development of a chicken experimental model platform for induced pluripotent stem cells by using CRISPR/Cas9-mediated NANOG knock-in reporter DF1 cells
    Article Snippet: .. Chicken NANOG knock-in reporter DF1 cells were harvested by treatment with 0.05% (v/v) trypsin supplemented with 0.53 mM EDTA and electroporated with each of the 4 reprogramming vectors using a Basic Fibroblast Nucleofector kit (Lonza VPI-1002, Basel, Switzerland) according to the manufacturer's instructions. .. Briefly, each vector was mixed with 80 μL of basic nucleofector solution and 16 μL of supplement 1.

    Article Title: Research Note: Development of a chicken experimental model platform for induced pluripotent stem cells by using CRISPR/Cas9-mediated NANOG knock-in reporter DF1 cells.
    Article Snippet: .. Chicken NANOG knock-in reporter DF1 cells were harvested by treatment with 0.05% (v/v) trypsin supplemented with 0.53 mM EDTA and electroporated with each of the 4 reprogramming vectors using a Basic Fibroblast Nucleofector kit (Lonza VPI-1002, Basel, Switzerland) according to the manufacturer’s instructions. .. Briefly, each vector was mixed with 80 mL of basic nucleofector solution and 16 mL of supplement 1.



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    Image Search Results


    Journal: iScience

    Article Title: Exosomal release of the virus-encoded chemokine receptor US28 contributes to chemokine scavenging

    doi: 10.1016/j.isci.2023.107412

    Figure Lengend Snippet:

    Article Snippet: Amaxa basic fibroblast nucleofector kit , Lonza , Cat. #: VPI-1002.

    Techniques: Virus, Recombinant, Luciferase, Software